Detecting Validated Intracellular ROS Generation with ¹⁸F-dihydroethidine-Based PET.

ABSTRACT:

Purpose

To determine the sensitivity of the ¹⁸F-radiolabelled dihydroethidine analogue ([¹⁸F]DHE) to ROS in a validated ex vivo model of tissue oxidative stress.

Procedures

The sensitivity of [¹⁸F]DHE to various ROS-generating systems was first established in vitro. Then, isolated rat hearts were perfused under constant flow, with contractile function monitored by intraventricular balloon. Cardiac uptake of infused [¹⁸F]DHE (50-150 kBq.min^-1) was monitored by γ-detection, while ROS generation was invoked by menadione infusion (0, 10, or 50 μm), validated by parallel measures of cardiac oxidative stress.

Results

[¹⁸F]DHE was most sensitive to oxidation by superoxide and hydroxyl radicals. Normalised [

SUBMITTER: Waters ECT 

PROVIDER: S-EPMC9085669 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature