Integrated transcriptome and endogenous hormone analysis provides new insights into callus proliferation in Osmanthus fragrans.

ABSTRACT: Osmanthus fragrans is an important evergreen species with both medicinal and ornamental value in China. Given the low efficiency of callus proliferation and the difficulty of adventitious bud differentiation, tissue culture and regeneration systems have not been successfully established for this species. To understand the mechanism of callus proliferation, transcriptome sequencing and endogenous hormone content determination were performed from the initial growth stages to the early stages of senescence on O. fragrans calli. In total, 47,340 genes were identified by transcriptome sequencing, including 1798 previously unidentified genes specifically involved in callus development. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of differentially expressed genes (DEGs) was si

SUBMITTER: Gu H 

PROVIDER: S-EPMC9085794 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature