Quantitative proteomics reveals specific protein regulation of severe hypospadias.

ABSTRACT:

Background

The etiological mechanism of hypospadias is multifactorial and may be heterogeneous by severity. To date, very limited analyses on proteome in hypospadias have been conducted, and there are still no severe hypospadias proteomics analyses.

Methods

In our study, tandem mass tag (TMT)-based quantitative proteomics was performed, exploring the clinical samples from hypospadias patients and healthy donators, in order to identify distinctly expressed proteins for severe hypospadias. To further uncover the mechanistic links in these complex proteomics data, we performed several core ingenuity pathway analyses (IPA) to predict, based on these observed different expression of proteins (DEPs).

Results

Compared with the unaffected controls, 299 proteins were found to

SUBMITTER: Zhu S 

PROVIDER: S-EPMC9085921 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature