Molecular cloning, characterization, and functional analysis of the uncharacterized C11orf96 gene.

ABSTRACT:

Background

The mammalian genome encodes millions of proteins. Although many proteins have been discovered and identified, a large part of proteins encoded by genes are yet to be discovered or fully characterized. In the present study, we successfully identified a host protein C11orf96 that was significantly upregulated after viral infection.

Results

First, we successfully cloned the coding sequence (CDS) region of the cat, human, and mouse C11orf96 gene. The CDS region of the C11orf96 gene is 372 bp long, encodes 124 amino acids, and is relatively conserved in different mammals. From bioinformatics analysis, we found that C11orf96 is rich in Ser and has multiple predicted phosphorylation sites. Moreover, protein interaction prediction analysis revealed that the protein is as

SUBMITTER: Yang H 

PROVIDER: S-EPMC9086667 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature