Simultaneous analysis of antigen-specific B and T cells after SARS-CoV-2 infection and vaccination.

ABSTRACT: Conventional methods for quantifying and phenotyping antigen-specific lymphocytes can rapidly deplete irreplaceable specimens. This is due to the fact that antigen-specific T and B cells have historically been analyzed in independent assays each requiring millions of cells. A technique that facilitates the simultaneous detection of antigen-specific T and B cells would allow for more thorough immune profiling with significantly reduced sample requirements. To this end, we developed the B and T cell tandem lymphocyte evaluation (BATTLE) assay, which allows for the simultaneous identification of SARS-CoV-2 Spike reactive T and B cells using an activation induced marker (AIM) T cell assay and dual-color B cell antigen probes. Using this assay, we demonstrate that antigen-specific B and T cell

SUBMITTER: Newell KL 

PROVIDER: S-EPMC9087145 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature