A Sensitive and Controlled Data-Independent Acquisition Method for Proteomic Analysis of Cell Therapies.

ABSTRACT: Mass spectrometry (MS)-based proteomic measurements are uniquely poised to impact the development of cell and gene therapies. With the adoption of rigorous instrumental performance qualifications (PQs), large-scale proteomics can move from a research to a manufacturing control tool. Especially suited, data-independent acquisition (DIA) approaches have distinctive qualities to extend multiattribute method (MAM) principles to characterize the proteome of cell therapies. Here, we describe the development of a DIA method for the sensitive identification and quantification of proteins on a Q-TOF instrument. Using the improved acquisition parameters, we defined a control strategy and highlighted some metrics to improve the reproducibility of SWATH acquisition-based proteomic measurements. Finall

SUBMITTER: Lombard-Banek C 

PROVIDER: S-EPMC9087334 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature