Development of a portable electrochemical loop mediated isothermal amplification (LAMP) device for detection of hepatitis B virus.

ABSTRACT: The objective of this study was to develop a simple, inexpensive prototype device for rapid detection of hepatitis B virus (HBV). The device was able to simultaneously amplify, detect and quantify the target HBV DNA. The system was fabricated from a custom-made electrochemical set-up of which the temperature was thermostatically controlled by a water bath. Real-time monitoring of HBV DNA was accomplished by measuring the response of redox indicator in the reaction mixture. Concentration of HBV DNA in the samples was determined from the peak high ratio (PHR) and threshold time relationship. The signal was processed by sigmoidal model fitting to enhance the accuracy of the results. Key parameters including concentrations of redox indicator and reaction temperatures were optimized. Sensitivit

SUBMITTER: Jayanath NY 

PROVIDER: S-EPMC9087361 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature