Exchanging the order of carotenogenic genes linked by porcine teschovirus-1 2A peptide enable to optimize carotenoid metabolic pathway in Saccharomyces cerevisiae.

ABSTRACT: The yeast Saccharomyces cerevisiae serves as a promising host for the production of a wide range of chemical compounds and fuels. Currently, simultaneous expression of several genes could be achieved via the use of 2A viral peptides, yet detailed characterizations to assess the discrepancy of different orders of genes linked by 2A peptides are rarely sufficient. In this study, we investigated the effects of the order of genes linked by porcine teschovirus-1 2A (P2A) peptide on the metabolic pathway in S. cerevisiae. A heterologous carotenoid biosynthetic system involving nine kinds of polycistronic expression of codon-optimized carotenogenic genes GGPPS, CARB and CARRP from Blakeslea trispora was introduced into S. cerevisiae. The ord

SUBMITTER: Jiao X 

PROVIDER: S-EPMC9087642 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature