Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet.

ABSTRACT: We have developed a sheet-like pH imaging sensor based on a flexible and physically adhesive polymer thin film (referred to as a "pH sensing nanosheet"). The pH sensing nanosheet was composed of two films: one is a pH-sensitive layer-by-layer (LbL) film constructed from fluorescein-conjugated poly(acrylic acid) and poly(allylamine hydrochloride) and the other is a pH-insensitive film made from Nile red-embedded poly(d,l-lactic acid). The pH sensing nanosheet enabled the ratiometric imaging of pH changes in a leaf (500 × 500 μm²), namely the apoplastic ion milieu responding to an external NaCl stress. It was successfully mapped out that the alkalization of the leaf apoplast spread from the leaf base to the tip at 20 min after the stimulation and the pH value increased up to appro

SUBMITTER: Someya D 

PROVIDER: S-EPMC9087818 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature