Single-cell profiling of human dura and meningioma reveals cellular meningeal landscape and insights into meningioma immune response.

ABSTRACT:

Background

Recent investigations of the meninges have highlighted the importance of the dura layer in central nervous system immune surveillance beyond a purely structural role. However, our understanding of the meninges largely stems from the use of pre-clinical models rather than human samples.

Methods

Single-cell RNA sequencing of seven non-tumor-associated human dura samples and six primary meningioma tumor samples (4 matched and 2 non-matched) was performed. Cell type identities, gene expression profiles, and T cell receptor expression were analyzed. Copy number variant (CNV) analysis was performed to identify putative tumor cells and analyze intratumoral CNV heterogeneity. Immunohistochemistry and imaging mass cytometry was performed on selected samples to validate pro

SUBMITTER: Wang AZ 

PROVIDER: S-EPMC9088131 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature