PGK1-coupled HSP90 stabilizes GSK3β expression to regulate the stemness of breast cancer stem cells.

ABSTRACT:

Objective

Glycogen synthase kinase-3β (GSK3β) has been recognized as a suppressor of Wnt/β-catenin signaling, which is critical for the stemness maintenance of breast cancer stem cells. However, the regulatory mechanisms of GSK3β protein expression remain elusive.

Methods

Co-immunoprecipitation and mass spectral assays were performed to identify molecules binding to GSK3β, and to characterize the interactions of GSK3β, heat shock protein 90 (Hsp90), and co-chaperones. The role of PGK1 in Hsp90 chaperoning GSK3β was evaluated by constructing 293T cells stably expressing different domains/mutants of Hsp90α, and by performing a series of binding assays with bacterially purified proteins and clinical specimens. The influences of Hsp90 inhibitors on breast cancer stem cell stemne

SUBMITTER: Tang W 

PROVIDER: S-EPMC9088184 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature