Pt-grown carbon nanofibers for enzymatic glutamate biosensors and assessment of their biocompatibility.

ABSTRACT: Application-specific carbon nanofibers grown from Pt-catalyst layers have been shown to be a promising material for biosensor development. Here we demonstrate immobilization of glutamate oxidase on them and their use for amperometric detection of glutamate at two different potentials. At -0.15 V vs. Ag/AgCl at concentrations higher than 100 μM the oxygen reduction reaction severely interferes with the enzymatic production of H₂O₂ and consequently affects the detection of glutamate. On the other hand, at 0.6 V vs. Ag/AgCl enzyme saturation starts to affect the measurement above a glutamate concentration of 100 μM. Moreover, we suggest here that glutamate itself might foul Pt surfaces to some degree, which should be taken into account when designing Pt-bas

SUBMITTER: Isoaho N 

PROVIDER: S-EPMC9088215 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature