A pyrosequencing protocol for rapid identification of SARS-CoV-2 variants.

ABSTRACT: Next-generation sequencing (NGS) is the primary method used to monitor the distribution and emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants around the world; however, it is costly and time-consuming to perform and is not widely available in low-resourced geographical regions. Pyrosequencing has the potential to augment surveillance efforts by providing information on specific targeted mutations for rapid identification of circulating and emerging variants. The current study describes the development of a reverse transcription (RT)-PCR-pyrosequencing assay targeting >65 spike protein gene (S) mutations of SARS-CoV-2, which permits differentiation of commonly reported variants currently circulating in the United States with a high degree of confidence. Vari

SUBMITTER: Allen RA 

PROVIDER: S-EPMC9088475 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature