Towards understanding the formation of internal fragments generated by collisionally activated dissociation for top-down mass spectrometry.

ABSTRACT: Top-down mass spectrometry (TD-MS) generates fragment ions that returns information on the polypeptide amino acid sequence. In addition to terminal fragments, internal fragments that result from multiple cleavage events can also be formed. Traditionally, internal fragments are largely ignored due to a lack of available software to reliably assign them, mainly caused by a poor understanding of their formation mechanism. To accurately assign internal fragments, their formation process needs to be better understood. Here, we applied a statistical method to compare fragmentation patterns of internal and terminal fragments of peptides and proteins generated by collisionally activated dissociation (CAD). Internal fragments share similar fragmentation propensities with terminal fragments (e.g., e

SUBMITTER: Wei B 

PROVIDER: S-EPMC9088748 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature