Boron NMR as a Method to Screen Natural Product Libraries for B-Containing Compounds.

ABSTRACT: Natural products are biologically relevant metabolites exploited for biomedicine and biotechnology. The frequent reisolation of known natural products questions whether existing discovery models are still capable of identifying novel compounds. As innovative NMR-based screening techniques can help overcome these challenges, we applied a phase cycling composite pulse sequence to ¹¹B NMR experiments to enhance their sensitivity to screen libraries for novel boron-containing molecules. Aplasmomycin and autoinducer-2 were detected in crude and enhanced microbial fractions, via their boron signals, as proof of concept. Subsequently, a screen of 21 crude plant and 50 crude marine microbial extracts were chosen at random and analyzed with the optimized ¹¹B experiment for fea

SUBMITTER: Macho JM 

PROVIDER: S-EPMC9088847 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature