Dissecting Intra-Tumoral Changes Following Immune Checkpoint Blockades in Intrahepatic Cholangiocarcinoma via Single-Cell Analysis.

ABSTRACT:

Purpose

To dissect the tumor ecosystem following immune checkpoint blockades (ICBs) in intrahepatic cholangiocarcinoma (ICC) at a single-cell level.

Methods

Single-cell RNA sequencing (scRNA-seq) data of 10 ICC patients for the ICB clinical trial were extracted from GSE125449 and systematically reanalyzed. Bulk RNA-seq data of 255 ICC patients were analyzed. Infiltration levels of SPP1⁺CD68⁺ tumor-associated macrophages (TAMs) were examined by dual immunofluorescence (IF) staining in 264 resected ICC samples. The correlation between SPP1⁺ TAMs and clinicopathological features as well as their prognostic significance was evaluated.

Results

Among the 10 patients, five received biopsy at baseline, and others were biopsied at different tim

SUBMITTER: Sun BY 

PROVIDER: S-EPMC9088915 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature