Characterization of the Interfacial Liquid Layer Formed on Hydrophobic Packing Material Surfaces by Liquid Chromatographic Analysis of the Distribution of Ions and Molecules.

ABSTRACT: We determine the bulk liquid phase volumes in octadecyl-bonded silica (C₁₈ silica) columns equilibrated with acetonitrile-water and methanol-water (0-19%(v/v)) binary mixed solvents by a liquid chromatographic method with inorganic ions used as probes. The solvent composition and the thickness of the interfacial liquid layer formed on the C₁₈-bonded silica surface are then determined from the bulk liquid phase volume, the total liquid phase volume, the surface area of the C₁₈ silica packing material, and the retention volumes of the isotopically labeled eluent components for the columns. We used two C₁₈ silica packing materials having identical bonding structures but different pore sizes and surface areas. Our results show that various hydrophili

SUBMITTER: Shibukawa M 

PROVIDER: S-EPMC9089683 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature