Use of standard U-bottom and V-bottom well plates to generate neuroepithelial embryoid bodies.

ABSTRACT: The use of organoids has become increasingly popular recently due to their self-organizing abilities, which facilitate developmental and disease modeling. Various methods have been described to create embryoid bodies (EBs) generated from embryonic or pluripotent stem cells but with varying levels of differentiation success and producing organoids of variable size. Commercial ultra-low attachment (ULA) V-bottom well plates are frequently used to generate EBs. These plates are relatively expensive and not as widely available as standard concave well plates. Here, we describe a cost-effective and low labor-intensive method that creates homogeneous EBs at high yield in standard V- and U-bottom well plates by applying an anti-adherence solution to reduce surface attachment, followed by centrifu

SUBMITTER: Choy Buentello D 

PROVIDER: S-EPMC9089918 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature