Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis.

ABSTRACT: Cell mass and composition change with cell cycle progression. Our previous work characterized buoyant mass dynamics in mitosis (Miettinen et al., 2019), but how dry mass and cell composition change in mitosis has remained unclear. To better understand mitotic cell growth and compositional changes, we develop a single-cell approach for monitoring dry mass and the density of that dry mass every ~75 s with 1.3% and 0.3% measurement precision, respectively. We find that suspension grown mammalian cells lose dry mass and increase dry mass density following mitotic entry. These changes display large, non-genetic cell-to-cell variability, and the changes are reversed at metaphase-anaphase transition, after which dry mass continues accumulating. The change in dry mass density causes buoyant and dr

SUBMITTER: Miettinen TP 

PROVIDER: S-EPMC9090323 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature