Neuroanatomical Basis for the Orexinergic Modulation of Anesthesia Arousal and Pain Control.

ABSTRACT: Hypothalamic orexin (hypocretin) neurons play crucial roles in arousal control. Their involvement in anesthesia and analgesia remains to be better understood. In order to enhance our view on the neuroanatomy, we systematically mapped the projections of orexin neurons with confocal microscope and light sheet microscope. We specifically expressed optogenetic opsins tagged with fluorescence markers in orexin neurons through adeno-associated viral infection in the mouse brain. The imaging results revealed fine details and novel features of the orexin projections throughout the brain, particularly related to the nuclei regulating arousal and pain. We then optogenetically activated orexin neurons in the lateral hypothalamus to study the effects on anesthesia-related behaviors. cFos staining show

SUBMITTER: Xiang X 

PROVIDER: S-EPMC9090436 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature