A highly sensitive and selective fluorescent probe without quencher for detection of Pb²⁺ ions based on aggregation-caused quenching phenomenon.

ABSTRACT: Lead is a highly toxic heavy metal, and various functional nucleic acid (FNA)-based biosensors have been developed for the detection of Pb²⁺ in environmental monitoring. However, most fluorescence biosensors that have been reported were designed on the basis of a double-labeled (fluorophore and quencher group) DNA sequence, which not only involved an inconvenient organic synthesis but also restricted their wider use in practical applications. Here, we utilized a G-rich DNA sequence as a recognition probe and conjugated fluorene (CF) to develop a fluorescence sensor without a quencher based on the aggregation-caused quenching (ACQ) effect. In the presence of Pb²⁺, the degree of aggregation of CF was reduced because Pb²⁺ induced the formation of a G-quadruple

SUBMITTER: Li Q 

PROVIDER: S-EPMC9090614 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature