An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation.

ABSTRACT: Even though a detailed understanding of the proliferative characteristics of T lymphocytes is imperative in many research fields, prior studies have never reached a consensus on these characteristics, and on the corresponding cell cycle kinetics specifically. In this study, the general proliferative response of human T lymphocytes to phytohaemagglutinin (PHA) stimulation was characterized using a carboxyfluorescein succinimidyl ester-based flow cytometric assay. We were able to determine when PHA-stimulated T lymphocytes complete their first division, the proportion of cells that initiate proliferation, the subsequent division rate of the cells, and the impact of irradiation on these proliferative properties. Next, we accurately visualized the cell cycle progression of dividing T lymphocyt

SUBMITTER: Duthoo E 

PROVIDER: S-EPMC9090834 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature