Cullin-5 mutants reveal collective sensing of the nucleocytoplasmic ratio in Drosophila embryogenesis.

ABSTRACT: In most metazoans, early embryonic development is characterized by rapid division cycles that pause before gastrulation at the midblastula transition (MBT).¹ These cleavage divisions are accompanied by cytoskeletal rearrangements that ensure proper nuclear positioning. However, the molecular mechanisms controlling nuclear positioning are not fully elucidated. In Drosophila, early embryogenesis unfolds in a multinucleated syncytium. Nuclei rapidly move across the anterior-posterior (AP) axis at cell cycles 4-6 in a process driven by actomyosin contractility and cytoplasmic flows.^2,3 In shackleton (shkl) mutants, this axial spreading is impaired.⁴ Here, we show that shkl mutants carry mutations in the cullin-5 (cul-5) gene. Live imaging

SUBMITTER: Hayden L 

PROVIDER: S-EPMC9090985 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature