Astrocytic GABA transporter controls sleep by modulating GABAergic signaling in Drosophila circadian neurons.

ABSTRACT: A precise balance between sleep and wakefulness is essential to sustain a good quality of life and optimal brain function. GABA is known to play a key and conserved role in sleep control, and GABAergic tone should, therefore, be tightly controlled in sleep circuits. Here, we examined the role of the astrocytic GABA transporter (GAT) in sleep regulation using Drosophila melanogaster. We found that a hypomorphic gat mutation (gat^33-1) increased sleep amount, decreased sleep latency, and increased sleep consolidation at night. Interestingly, sleep defects were suppressed when gat^33-1 was combined with a mutation disrupting wide-awake (wake), a gene that regulates the cell-surface levels of the GABA_A receptor resistance to dieldrin (RDL) in the wake-promoting l

SUBMITTER: Chaturvedi R 

PROVIDER: S-EPMC9090989 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature