Cholesterol-sensing role of phenylalanine in the interaction of human islet amyloid polypeptide with lipid bilayers.

ABSTRACT: The interactions between hIAPP and the pancreatic β-cells are associated with β-cell death in type II diabetes. Cholesterol modulates hIAPP-membrane interaction and hIAPP aggregation. The molecular mechanism underlying this is not well understood. Here we explore the cholesterol-sensing role of F15 in the interactions of hIAPP and hIAPP_1-19 with various compositions of lipids, including DOPC, DPPC and DOPC/DPPC using NMR, CD, ThT fluorescence and dye leakage assays. We show that both hIAPP and hIAPP_1-19 are more potent in the disruption to the membranes with cholesterol than they are in the disruption to the membranes without cholesterol. A substitution of F15 by leucine affects the binding and disruption of the peptides to the membranes slightly in the absence of cho

SUBMITTER: Hao R 

PROVIDER: S-EPMC9091444 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature