Multilaboratory assessment of metagenomic next-generation sequencing for unbiased microbe detection.

ABSTRACT:

Introduction

Metagenomic next-generation sequencing (mNGS) assay for detecting infectious agents is now in the stage of being translated into clinical practice. With no approved approaches or guidelines available, laboratories adopt customized mNGS assays to detect clinical samples. However, the accuracy, reliability, and problems of these routinely implemented assays are not clear.

Objectives

To evaluate the performance of 90 mNGS laboratories under routine testing conditions through analyzing identical samples.

Methods

Eleven microbial communities were generated using 15 quantitative microbial suspensions. They were used as reference materials to evaluate the false negatives and false positives of participating mNGS protocols, as well as the ability to distinguish g

SUBMITTER: Han D 

PROVIDER: S-EPMC9091723 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature