Mitochondrial GCN5L1 regulates glutaminase acetylation and hepatocellular carcinoma.

ABSTRACT:

Background

Glutaminolysis is a critical metabolic process that promotes cancer cell proliferation, including hepatocellular carcinoma (HCC). Delineating the molecular control of glutaminolysis could identify novel targets to ameliorate this oncogenic metabolic pathway. Here, we evaluated the role of general control of amino acid synthesis 5 like 1 (GCN5L1), a regulator of mitochondrial protein acetylation, in modulating the acetylation and activity of glutaminase to regulate HCC development.

Methods

Cell proliferation was determined by MTT, 2D and soft agar clone formation assays and orthotopic tumour assays in nude mice. GLS1/2 acetylation and activities were measured in cells and tumours to analyse the correlation with GCN5L1 expression and mTORC1 activation.

Results

SUBMITTER: Zhang T 

PROVIDER: S-EPMC9091986 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature