Autoantigen presentation by splenic dendritic cells is required for RBC-specific autoimmunity.

ABSTRACT:

Background

Failure of humoral tolerance to red blood cell (RBC) antigens may lead to autoimmune hemolytic anemia (AIHA), a severe and sometimes fatal disease. Previous studies have shown that although tolerance is robust in HOD mice, autoantibodies are generated upon adoptive transfer of OTII CD4⁺ T cells, which are specific for an epitope contained within the HOD antigen. These data imply that antigen-presenting cells (APCs) are presenting RBC-derived autoantigen(s) and are capable of driving T-cell activation. Given that multiple APCs participate in erythrophagocytosis, we used a transgenic approach to determine which cellular subsets were required for autoantigen presentation and subsequent autoreactive T-cell activation.

Study design and methods

HOD mice, which express an RBC-specific antigen consisting of hen egg lysozyme, ovalbumin, and human blood group molecule Duffy, were bred with IAb^fl/fl and Cre-expressing transgenic animals to generate mice that lack I-A^b expression on particular cell subsets. OTII CD4⁺ T cell proliferation was assessed in vivo in HOD⁺ I-Ab^fl/fl xCre⁺ mice and in vitro upon coculture with sorted APCs.

Results

Analysis of HOD⁺ I-Ab^fl/fl xCre⁺ mice demonstrated that splenic conventional dendritic cells (DCs), but not macrophages or monocytes, were required for autoantigen presentation to OTII CD4⁺ T cells. Subsequent in vitro coculture experiments revealed that both CD8⁺ and CD8^- DC subsets participate in erythrophagocytosis, present RBC-derived autoantigen and stimulate autoreactive T-cell proliferation.

Conclusion

These data suggest that if erythrocyte T-cell tolerance fails, DCs are capable of initiating autoimmune responses. As such, targeting DCs may be a fruitful strategy for AIHA therapies.

SUBMITTER: Richards AL 

PROVIDER: S-EPMC9092285 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature