A Ratiometric Fluorescence Probe for Selective Detection of ex vivo Methylglyoxal in Diabetic Mice.

ABSTRACT: Accurate monitoring of methylglyoxal (MGO) at cell and living level was crucial to reveal its role in the pathogenesis of diabetes since MGO was closely related to diabetes. Herein, a ratiometric fluorescence strategy was constructed based on the capture probe 2,3-diaminonaphthalene (DAN) for the specific detection of MGO. Compared to the fluorescent probes with a single emission wavelength, the ratiometric mode by monitoring two emissions can effectively avoid the interference from the biological background, and provided additional self-calibration ability, which can realize accurate detection of MGO. The proposed method showed a good linear relationship in the range of 0-75 μm for MGO detection, and the limit of detection was 0.33 μm. DAN responded to MGO with good specificity and was su

SUBMITTER: Xie Q 

PROVIDER: S-EPMC9092288 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature