Extracellular CIRP and TREM-1 axis promotes ICAM-1-Rho-mediated NETosis in sepsis.

ABSTRACT: Extracellular cold-inducible RNA-binding protein (eCIRP) is a damage-associated molecular pattern (DAMP). Intercellular adhesion molecule-1 (ICAM-1) expressing neutrophils produce excessive amounts of neutrophil extracellular traps (NETs). We reveal that eCIRP generates ICAM-1⁺ neutrophils through triggering receptor expressed on myeloid cells-1 (TREM-1) and the ICAM-1⁺ neutrophils involve Rho GTPase to promote NETosis. Treatment of BMDN with rmCIRP increased the frequency of ICAM-1⁺ BMDN, while rmCIRP-treated TREM-1^-/- BMDN or pretreatment of BMDN with TREM-1 inhibitor LP17 significantly decreased the frequency of ICAM-1⁺ neutrophils. The frequencies of ICAM-1⁺ neutrophils in blood and lungs were markedly decreased in rmCIR

SUBMITTER: Murao A 

PROVIDER: S-EPMC9092350 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature