Ti₃C₂ MXene-anchored photoelectrochemical detection of exosomes by in situ fabrication of CdS nanoparticles with enzyme-assisted hybridization chain reaction.

ABSTRACT: Exosomes that carry large amounts of tumor-specific molecular information have been identified as a potential non-invasive biomarker for early warning of cancer. In this work, we reported an enzyme-assisted photoelectrochemical (PEC) biosensor for quantification of exosomes based on the in situ synthesis of Ti₃C₂ MXene/CdS composites with magnetic separation technology and hybridization chain reaction (HCR). First, exosomes were specifically bound between aptamer-labeled magnetic beads (CD63-MBs) and a cholesterol-labeled DNA anchor. The properly designed anchor ends acted as a trigger to enrich the alkaline phosphatase (ALP) through HCR. It catalyzed more sodium thiophosphate to generate the sulfideion (S^2-), which combined with Cd²⁺ for

SUBMITTER: Qiu Z 

PROVIDER: S-EPMC9092378 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature