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Towards identification of protein-protein interaction stabilizers via inhibitory peptide-fragment hybrids using templated fragment ligation.

ABSTRACT: Using the hDMX/14-3-3 interaction, acylhydrazone-based ligand-directed fragment ligation was used to identify protein-protein interaction (PPI) inhibitory peptide-fragment hybrids. Separation of the peptide-fragment hybrids into the components yielded fragments that stabilized the hDMX/14-3-3 interaction.

SUBMITTER: Srdanovic S

PROVIDER: S-EPMC9092428 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Towards identification of protein-protein interaction stabilizers via inhibitory peptide-fragment hybrids using templated fragment ligation.

Srdanović Sonja S Hegedüs Zsofia Z Warriner Stuart L SL Wilson Andrew J AJ

RSC chemical biology 20220401 5

Using the hDMX/14-3-3 interaction, acylhydrazone-based ligand-directed fragment ligation was used to identify protein-protein interaction (PPI) inhibitory peptide-fragment hybrids. Separation of the peptide-fragment hybrids into the components yielded fragments that stabilized the hDMX/14-3-3 interaction. ...[more]

PMID: 35656480

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