Graphite nanocrystals coated paper-based electrode for detection of SARS-Cov-2 gene using DNA-functionalized Au@carbon dot core-shell nanoparticles.

ABSTRACT: Currently, the development of biosensors is an urgent need due to the rapid spread of SARS-CoV-2 and the limitations of current standard methods for the diagnosis of COVID-19. Hence, many researchers have focused on the design of high-performance biosensors for measuring coronavirus genes. In this study, a voltammetric genosensor was developed for the determination of SARS-CoV-2 RdRP gene based on the format of cDNA probe/Au@CD core-shell NPs/graphite nanocrystals (GNCs)/paper electrode. For the first time, graphite nanocrystals were used in the electrochemical biosensor design. This genosensor was exposed to different concentrations of virus gene and then the hybridization between cDNA probe and RdRP gene was monitored by redox-active toluidine blue (TB). With increasing the RdRP concentr

SUBMITTER: Ali Farzin M 

PROVIDER: S-EPMC9093088 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature