ABSTRACT: A 2-year (2017-2018) field experiment was performed to specify if the foliar application of putrescine (PUT) under optimum and water-deficit stress (WDS) conditions would favorably affect leaf gas exchange, greenness, chlorophyll fluorescence parameters, pigments, sodium (Na), potassium (K), as well as yield and content of the essential oil (EO) relationships in Salvia officinalis L. (sage) in spring (cutting 1) and summer (cutting 2). Based on the results analysis of variance, the effects of WDS, PUT, and cutting time were significant for the dry weight, leaf area index (LAI), EO content, EO yield, chlorophyll (Chl) t, carotenoid, Na, and K of sage. According to regression results, the response of EO content, EO yield, non-photochemical quenching (NPQ), spad, Chl a, Chl t, K, and K