Population distributions of single-cell adhesion parameters during the cell cycle from high-throughput robotic fluidic force microscopy.

ABSTRACT: Single-cell adhesion plays an essential role in biological and biomedical sciences, but its precise measurement for a large number of cells is still a challenging task. At present, typical force measuring techniques usually offer low throughput, a few cells per day, and therefore are unable to uncover phenomena emerging at the population level. In this work, robotic fluidic force microscopy (FluidFM) was utilized to measure the adhesion parameters of cells in a high-throughput manner to study their population distributions in-depth. The investigated cell type was the genetically engineered HeLa Fucci construct with cell cycle-dependent expression of fluorescent proteins. This feature, combined with the high-throughput measurement made it possible for the first time to characterize the sing

SUBMITTER: Nagy AG 

PROVIDER: S-EPMC9095720 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature