Trichosanthin inhibits cell growth and metastasis by promoting pyroptosis in non-small cell lung cancer.

ABSTRACT:

Background

A number of studies have demonstrated that trichosanthin (TCS) can induce apoptosis in numerous types of tumor cell lines. However, whether TCS can induce pyroptosis has not yet been reported. This study aimed to investigate the role of TCS and its inhibitory effect on tumor growth by modulating pyroptosis in non-small cell lung cancer (NSCLC).

Methods

Effects of different concentrations of TCS on the cell viability, proliferation, migration and invasion of NSCLC were detected by Cell Counting Kit-8 (CCK-8), colony formation, migration, and invasion assays. Immunofluorescence was used to detect the effect of TCS on the expression of pyroptosis marker protein gasdermin-D (GSDMD)-N in A549 cells. A tumor xenograft animal model was established by injecting A549 cells

SUBMITTER: Tan Y 

PROVIDER: S-EPMC9096284 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature