Effect of high-throughput screening of circRNA01724 on a rat model of myocardial ischemia ventricular arrhythmia.

ABSTRACT:

Background

It is considered that circRNA can participate in regulating the occurrence and effects of ventricular arrhythmia (VA) through competing endogenous RNA (ceRNA) mechanism, regulating pre-mRNA and regulating parental gene expression. Therefore, we used animal modeling and high-throughput differential screening to screen out circRNA related to VA and study its possible mechanism of action on VA.

Methods

The rat model of myocardial ischemia VA was established. High-throughput screening of the differentiated circRNA was conducted and verified by real-time quantitative polymerase chain reaction (qRT-PCR) and Western blot. Lv-circRNA01724 lentivirus was constructed using molecular biology. Primary isolation of the rat cardiomyocytes, hypoxia modeling, Lv-circRNA01724 tran

SUBMITTER: Hu X 

PROVIDER: S-EPMC9096315 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature