Melatonin exerts anti-angiogenic and anti-inflammatory effects in alkali-burned corneas.

ABSTRACT:

Background

Corneal neovascularization (CNV) caused by alkali burn injury is tightly associated with an inflammatory reaction and can lead to vision loss. Melatonin is involved in anti-inflammation and anti-angiogenesis, but its role in CNV has not yet been investigated.

Methods

We induced CNV using sodium hydroxide (NaOH) and compared the reactions of vehicle control and melatonin-treated male C57BL/6 mice at 7 and 14 days following the corneal burn. The infiltration of inflammatory cells and the expression of proangiogenic factors, chemokines, and inflammation-related molecules were quantified via immunohistochemical (IHC) analysis and quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR), respectively. Murine peritoneal macrophages were used i

SUBMITTER: Meng J 

PROVIDER: S-EPMC9096387 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature