Enhancing the Sensitivity of the Virus BioResistor by Overoxidation: Detecting IgG Antibodies.

ABSTRACT: The Virus BioResistor (VBR) is a biosensor capable of rapid and sensitive detection of small protein disease markers using a simple dip-and-read modality. For example, the bladder cancer-associated protein DJ-1 (22 kDa) can be detected in human urine within 1.0 min with a limit of detection (LOD) of 10 pM. The VBR uses engineered virus particles as receptors to recognize and selectively bind the protein of interest. These virus particles are entrained in a conductive poly(3,4-ethylenedioxythiophene) or PEDOT channel. The electrical impedance of the channel increases when the target protein is bound by the virus particles. But VBRs exhibit a sensitivity that is inversely related to the molecular weight of the protein target. Thus, large proteins, such as IgG antibodies (150 kDa), can be und

SUBMITTER: Bhasin A 

PROVIDER: S-EPMC9096803 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature