MEF2C Expression Is Regulated by the Post-transcriptional Activation of the METTL3-m⁶A-YTHDF1 Axis in Myoblast Differentiation.

ABSTRACT: N ⁶-methyladenosine (m⁶A) plays an essential role in regulating gene expression. However, the effect of m⁶A on skeletal myoblast differentiation and the underlying mechanisms are still unclear. Here, we ascertained mRNA m⁶A methylation exhibited declined changes during bovine skeletal myoblast differentiation, and both MEF2C mRNA expression and m⁶A levels were significantly increased during myoblast differentiation. We found that MEF2C with mutated m⁶A sites significantly inhibited myoblast differentiation compared with wild-type MEF2C. METTL3 promoted MEF2C protein expression through posttranscriptional modification in an m⁶A-YTHDF1-dependent manner. Moreover, MEF2C promoted the expres

SUBMITTER: Yang X 

PROVIDER: S-EPMC9096896 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature