Chronic cholesterol depletion increases F-actin levels and induces cytoskeletal reorganization via a dual mechanism.

ABSTRACT: Previous work from us and others has suggested that cholesterol is an important lipid in the context of the organization of the actin cytoskeleton. However, reorganization of the actin cytoskeleton upon modulation of membrane cholesterol is rarely addressed in the literature. In this work, we explored the signaling crosstalk between cholesterol and the actin cytoskeleton by using a high-resolution confocal microscopic approach to quantitatively measure changes in F-actin content upon cholesterol depletion. Our results show that F-actin content significantly increases upon chronic cholesterol depletion, but not during acute cholesterol depletion. In addition, utilizing inhibitors targeting the cholesterol biosynthetic pathway at different steps, we show that reorganization of the actin cyto

SUBMITTER: Sarkar P 

PROVIDER: S-EPMC9096963 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature