Nf-rnaSeqCount: A Nextflow pipeline for obtaining raw read counts from RNA-seq data.

ABSTRACT: The rate of raw sequence production through Next-Generation Sequencing (NGS) has been growing exponentially due to improved technology and reduced costs. This has enabled researchers to answer many biological questions through "multi-omics" data analyses. Even though such data promises new insights into how biological systems function and understanding disease mechanisms, computational analyses performed on such large datasets comes with its challenges and potential pitfalls. The aim of this study was to develop a robust portable and reproducible bioinformatic pipeline for the automation of RNA sequencing (RNA-seq) data analyses. Using Nextflow as a workflow management system and Singularity for application containerisation, the nf-rnaSeqCount pipeline was developed for mapping raw RNA-seq

SUBMITTER: Mpangase PT 

PROVIDER: S-EPMC9097006 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature