Elucidating Film Loss and the Role of Hydrogen Bonding of Adsorbed Redox Enzymes by Electrochemical Quartz Crystal Microbalance Analysis.

ABSTRACT: The immobilization of redox enzymes on electrodes enables the efficient and selective electrocatalysis of useful reactions such as the reversible interconversion of dihydrogen (H₂) to protons (H⁺) and formate to carbon dioxide (CO₂) with hydrogenase (H₂ase) and formate dehydrogenase (FDH), respectively. However, their immobilization on electrodes to produce electroactive protein films for direct electron transfer (DET) at the protein-electrode interface is not well understood, and the reasons for their activity loss remain vague, limiting their performance often to hour timescales. Here, we report the immobilization of [NiFeSe]-H₂ase and [W]-FDH from Desulfovibrio vulgaris Hildenborough on a range of charged and neutral self-ass

SUBMITTER: Badiani VM 

PROVIDER: S-EPMC9097293 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature