Detergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs.

ABSTRACT: Hybrid vesicles (HVs) that consist of mixtures of block copolymers and lipids are robust biomimetics of liposomes, providing a valuable building block in bionanotechnology, catalysis, and synthetic biology. However, functionalization of HVs with membrane proteins remains laborious and expensive, creating a significant current challenge in the field. Here, using a new approach of extraction with styrene-maleic acid (SMA), we show that a membrane protein (cytochrome bo ₃) directly transfers into HVs with an efficiency of 73.9 ± 13.5% without the requirement of detergent, long incubation times, or mechanical disruption. Direct transfer of membrane proteins using this approach was not possible into liposomes, suggesting that HVs are more amenable than liposomes to membrane pr

SUBMITTER: Catania R 

PROVIDER: S-EPMC9097535 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature