A longitudinal study of the pulmonary mycobiome in subjects with and without chronic obstructive pulmonary disease.

ABSTRACT:

Background

Few studies have examined the stability of the pulmonary mycobiome. We report longitudinal changes in the oral and pulmonary mycobiome of participants with and without COPD in a large-scale bronchoscopy study (MicroCOPD).

Methods

Repeated sampling was performed in 30 participants with and 21 without COPD. We collected an oral wash (OW) and a bronchoalveolar lavage (BAL) sample from each participant at two time points. The internal transcribed spacer 1 region of the ribosomal RNA gene cluster was PCR amplified and sequenced on an Illumina HiSeq sequencer. Differences in taxonomy, alpha diversity, and beta diversity between the two time points were compared, and we examined the effect of intercurrent antibiotic use.

Results

Sample pairs were dominated by Cand

SUBMITTER: Martinsen EMH 

PROVIDER: S-EPMC9098062 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature