Constructing Cell-Free Expression Systems for Low-Cost Access.

ABSTRACT: Cell-free systems for gene expression have gained attention as platforms for the facile study of genetic circuits and as highly effective tools for teaching. Despite recent progress, the technology remains inaccessible for many in low- and middle-income countries due to the expensive reagents required for its manufacturing, as well as specialized equipment required for distribution and storage. To address these challenges, we deconstructed processes required for cell-free mixture preparation and developed a set of alternative low-cost strategies for easy production and sharing of extracts. First, we explored the stability of cell-free reactions dried through a low-cost device based on silica beads, as an alternative to commercial automated freeze dryers. Second, we report the positive effe

SUBMITTER: Guzman-Chavez F 

PROVIDER: S-EPMC9098194 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature