Metabolite profiling and protein quantification to a large library of 96 horsegram (Macrotyloma uniflorum) germplasm.

ABSTRACT: The present study was framed for the assessment of metabolic diversity of 96 diverse horsegram lines derived from 700 germplasm accessions of Pan India. The nutritional component of horsegram germplasm was studied using nuclear magnetic resonance spectroscopy (¹H NMR) and micro-Kjeldal method. Differential range of protein content was found 13-40% in the selected panel. The related wild species Macrotyloma sar-gharwalensis contained highest protein content (40%), and the lowest protein content (13%) was found in IC-120837 and TCR-1439. Seed based ¹H NMR spectroscopy of horsegram discovered 45 different metabolites (17 amino acids, 7 flavonoids, 10 organic acids/phenolic acids/sugar alcohols/steroids, 7 carbohydrades/sugars, and 4 vitamins). The number of total metabol

SUBMITTER: Gautam M 

PROVIDER: S-EPMC9098527 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature