Efficient deformation mechanisms enable invasive cancer cells to migrate faster in 3D collagen networks.

ABSTRACT: Cancer cell migration is a widely studied topic but has been very often limited to two dimensional motion on various substrates. Indeed, less is known about cancer cell migration in 3D fibrous-extracellular matrix (ECM) including variations of the microenvironment. Here we used 3D time lapse imaging on a confocal microscope and a phase correlation method to follow fiber deformations, as well as cell morphology and live actin distribution during the migration of cancer cells. Different collagen concentrations together with three bladder cancer cell lines were used to investigate the role of the metastatic potential on 3D cell migration characteristics. We found that grade-3 cells (T24 and J82) are characterized by a great diversity of shapes in comparison with grade-2 cells (RT112). Moreove

SUBMITTER: Laforgue L 

PROVIDER: S-EPMC9098560 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature