Acute Increase of Renal Perfusion Pressure Causes Rapid Activation of mTORC1 (Mechanistic Target Of Rapamycin Complex 1) and Leukocyte Infiltration.

ABSTRACT:

Background

The present study in Sprague-Dawley rats determined the effects of a rapid rise of renal perfusion pressure (RPP) upon the activation of mTOR (mechanistic target of rapamycin), and the effects upon the infiltration of CD68-positive macrophages/monocytes and CD3-positive T lymphocytes into the kidneys.

Methods

RPP was elevated by 40 mm Hg for 30 minutes in male Sprague-Dawley rats while measuring renal blood flow and urine flow rate. Sham rats were studied in the same way, but RPP was not changed. Since initial studies found that the acute increase of RPP resulted in activation of mTORC1 (phosphorylation of S6^S235/236), the effects of inhibition of mTORC1 with rapamycin pretreatment were then determined.

Results

It was found that a 30-minute incre

SUBMITTER: Shimada S 

PROVIDER: S-EPMC9098670 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature