Characterization of RNA content in individual phase-separated coacervate microdroplets.

ABSTRACT: Condensates formed by complex coacervation are hypothesized to have played a crucial part during the origin-of-life. In living cells, condensation organizes biomolecules into a wide range of membraneless compartments. Although RNA is a key component of biological condensates and the central component of the RNA world hypothesis, little is known about what determines RNA accumulation in condensates and to which extend single condensates differ in their RNA composition. To address this, we developed an approach to read the RNA content from single synthetic and protein-based condensates using high-throughput sequencing. We find that certain RNAs efficiently accumulate in condensates. These RNAs are strongly enriched in sequence motifs which show high sequence similarity to short interspersed

SUBMITTER: Wollny D 

PROVIDER: S-EPMC9098875 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature